Araç Laboratory at UChicago

The adhesion GPCRs CELSR1-3 and LPHN3 engage G proteins via distinct activation mechanisms.
Bui DLH, Roach A, Li J, Bandekar SJ, Orput E, Raghavan R, Araç D, Sando RC.
Cell Rep. 2023 May 23;42(6):112552. doi: 10.1016/j.celrep.2023.112552. PMID: 37224017.

Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism
Li J, Xie Y, Cornelius S, Jiang X, Sando R, Kordon SP, Pan M, Leon K, Südhof TC, Zhao M, Araç D.
Nat Commun. 2020 May 1;11(1):2140. doi: 10.1038/s41467-020-16029-7. PMID: 32358586 

Structural basis for adhesion G protein-coupled receptor Gpr126 function
Leon K, Cunningham RL, Riback JA, Feldman E, Li J, Sosnick TR, Zhao M, Monk KR, Araç D.
Nat Commun. 2020 Jan 10;11(1):194. doi: 10.1038/s41467-019-14040-1. PMID: 31924782

Teneurin Structure: Splice Variants of a Bacterial Toxin Homolog Specifies Synaptic Connections
Araç D, Li J.
Front Neurosci. 2019 Aug 7;13:838. doi: 10.3389/fnins.2019.00838. eCollection 2019. PMID: 31440135  

Teneurins and latrophilins: two giants meet at the synapse
Araç D, Li J.
Curr Opin Struct Biol. 2019 Feb;54:141-151. doi: 10.1016/j.sbi.2019.01.028. Epub 2019 Apr 2.PMID: 30952063  

A Comprehensive Mutagenesis Screen of the Adhesion GPCR Latrophilin-1/ADGRL1
Nazarko O, Kibrom A, Winkler J, Leon K, Stoveken H, Salzman G, Merdas K, Lu Y, Narkhede P, Tall G, Prömel S, Araç D.
iScience. 2018 May 25;3:264-278. doi: 10.1016/j.isci.2018.04.019. Epub 2018 Apr 30. PMID: 30428326 

A new MR-SAD algorithm for the automatic building of protein models from low-resolution X-ray data and a poor starting model
Skubák P, Araç D, Bowler MW, Correia AR, Hoelz A, Larsen S, Leonard GA, McCarthy AA, McSweeney S, Mueller-Dieckmann C, Otten H, Salzman G, Pannu NS. NS.IUCrJ. 2018 Jan 25;5(Pt 2):166-171. doi: 10.1107/S2052252517017961. eCollection 2018 Mar 1.PMID: 29765606  

Structural Basis for Teneurin Function in Circuit-Wiring: A Toxin Motif at the Synapse
Li J., Shalev-Benami M., Sando R., Jiang X., Kibrom A., Wang J., Leon K., Katanski C., Nazarko O., Lu Y., Südhof TC., Skiniotis G., Araç D.
Cell. 2018 Apr 19;173(3):735-748.e15. doi: 10.1016/j.cell.2018.03.036. PMID: 29677516
UChicago News
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Salzman, GS., Zhang S., Gupta A., Koide A., Koide S., Araç D. Stachel-independent modulation of GPR56/ADGRG1 signaling by synthetic ligands directed to its extracellular region. PNAS. 2017. PMID: 28874577 

Salzman GS, Ackerman SD, Ding C, Koide A, Leon K, Luo R, Stoveken HM, Fernandez, CG, Tall GG, Piao X, Monk KR, Koide S, Araç D. Structural basis for regulation of GPR56/ADGRG1 by its alternatively spliced extracellular domains. Neuron. 2016 Sept 21. PMID: 27657451 UChicago News: https://www.uchicagomedicine.org/biological-sciences-articles/researcher… 

Lu YC, Nazarko OV, Sando R 3rd, Salzman GS, Südhof TC, Araç D. Structural Basis of Latrophilin-FLRT-UNC5 Interaction in Cell Adhesion. Structure. 2015 Jul 28. PMID: 26235030 

Hamann J, Aust G, Araç D, Engel FB, Formstone C, Fredriksson R, Hall RA, Harty BL, Kirchhoff C, Knapp B, Krishnan A, Liebscher I, Lin HH, Martinelli DC, Monk KR, Peeters MC, Piao X, Prömel S, Schöneberg T, Schwartz TW, Singer K, Stacey M, Ushkaryov YA, Vallon M, Wolfrum U, Wright MW, Xu L, Langenhan T, Schiöth HB. International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors. Pharmacol Rev. 2015 Apr; 67(2):338-67. PMID: 25713288 

Liebscher I, Ackley B, Araç D, Ariestanti DM, Aust G, Bae BI, Bista BR, Bridges JP, Duman JG, Engel FB, Giera S, Goffinet AM, Hall RA, Hamann J, Hartmann N, Lin HH, Liu M, Luo R, Mogha A, Monk KR, Peeters MC, Prömel S, Ressl S, Schiöth HB, Sigoillot SM, Song H, Talbot WS, Tall GG, White JP, Wolfrum U, Xu L, Piao X. New functions and signaling mechanisms for the class of adhesion G protein-coupled receptors. Ann N Y Acad Sci. 2014 Dec;1333:43-64. PMID: 25424900 

Tsetsenis T, Boucard AA, Araç D, Brunger AT, Südhof TC. Direct visualization of trans-synaptic neurexin-neuroligin interactions during synapse formation. J Neurosci. 2014 Nov 5;34(45):15083-96. PMID: 25378172 

Luo R, Jeong SJ, Yang A, Wen M, Saslowsky DE, Lencer WI, Araç D, Piao X. Mechanism for adhesion G protein-coupled receptor GPR56-mediated RhoA activation induced by collagen III stimulation. PLoS One. 2014 Jun 20;9(6):e100043. PMID: 24949629  

Prömel S, Langenhan T, Araç D. Matching structure with function: the GAIN domain of Adhesion-GPCR and PKD1-like proteins. Trends Pharmacol Sci. 2013 Aug; 34(8):470-478. PMID: 23850273 

Araç D, Aust G, Calebiro D, Engel FB, Formstone C, Goffinet A, Hamann J, Kittel RJ, Liebscher I, Lin HH, Monk KR, Petrenko A, Piao X, Prömel S, Schiöth HB, Schwartz TW, Stacey M, Ushkaryov YA, Wobus M, Wolfrum U, Xu L, Langenhan T. Dissecting signaling and functions of adhesion G protein-coupled receptors. Ann N Y Acad Sci. 2012 Dec; 1276:1-25. PMID: 23215895. 

Araç D, Boucard AA, Bolliger MF, Nguyen J, Soltis SM, Südhof TC, Brunger AT. A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. EMBO J. 2012 Mar 21; 31(6):1364-78. PMID: 22333914. 

Zhang C, Atasoy D, Araç D, Yang X, Fucillo MV, Robison AJ, Ko J, Brunger AT, Südhof TC. Neurexins physically and functionally interact with GABA(A) receptors. Neuron. 2010 May 13; 66(3):403-16. PMID: 20471353. 

Ko J, Zhang C, Arac D, Boucard AA, Brunger AT, Südhof TC. Neuroligin-1 performs neurexin-dependent and neurexin-independent functions in synapse validation. EMBO J. 2009 Oct 21; 28(20):3244-55. PMID: 19730411. 

Zuniga JE, Schmidt JJ, Fenn T, Burnett JC, Araç D, Gussio R, Stafford RG, Badie SS, Bavari S, Brunger AT. A potent peptidomimetic inhibitor of botulinum neurotoxin serotype A has a very different conformation than SNAP-25 substrate. Structure. 2008 Oct 8; 16(10):1588-97. PMID: 18940613. 

Araç D, Boucard AA, Özkan E, Strop P, Newell E, Südhof TC, Brunger AT. Structures of neuroligin-1 and the neuroligin-1/neurexin-1 beta complex reveal specific protein-protein and protein-Ca2+ interactions. Neuron. 2007 Dec 20; 56(6):992-1003. PMID: 18093522. 

Dai H, Shen N, Araç D, Rizo J. A quaternary SNARE-synaptotagmin-Ca2+-phospholipid complex in neurotransmitter release. J Mol Biol. 2007 Mar 30; 367(3):848-63. PMID: 17320903. 

Rizo J, Chen X, Araç D. Unraveling the mechanisms of synaptotagmin and SNARE function in neurotransmitter release. Trends Cell Biol. 2006 Jul; 16(7):339-50. PMID: 16698267. 

Li L, Shin OH, Rhee JS, Araç D, Rah JC, Rizo J, Südhof T, Rosenmund C. Phosphatidylinositol phosphates as co-activators of Ca2+ binding to C2 domains of synaptotagmin 1. J Biol Chem. 2006 Jun 9; 281(23):15845-52. PMID: 16595652. 

Chen X, Araç D, Wang TM, Gilpin CJ, Zimmerberg J, Rizo J. SNARE-mediated lipid mixing depends on the physical state of the vesicles. Biophys J. 2006 Mar 15; 90(6):2062-74. PMID: 16361343. 

Araç D, Chen X, Khant HA, Ubach J, Ludtke SJ, Kikkawa M, Johnson AE, Chiu W, Südhof TC, Rizo J. Close membrane-membrane proximity induced by Ca(2+)-dependent multivalent binding of synaptotagmin-1 to phospholipids. Nat Struct Mol Biol. 2006 Mar; 13(3):209-17. PMID: 16491093. 

Ferguson AD, Labunskyy VM, Fomenko DE, Araç D, Chelliah Y, Amezcua CA, Rizo J, Gladyshev VN, Deisenhofer J. NMR structures of the selenoproteins Sep15 and SelM reveal redox activity of a new thioredoxin-like family. J Biol Chem. 2006 Feb 10; 281(6):3536-43. PMID: 16319061. 

Araç D, Dulubova I, Pei J, Huryeva I, Grishin NV, Rizo J. Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5. J Mol Biol. 2005 Feb 18; 346(2):589-601. PMID: 15670607. 

Jarousse N, Wilson JD, Arac D, Rizo J, Kelly RB. Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motif. Traffic. 2003 Jul; 4(7):468-78. PMID: 12795692. 

Araç D, Murphy T, Rizo J. Facile detection of protein-protein interactions by one-dimensional NMR spectroscopy. Biochemistry. 2003 Mar 18; 42(10):2774-80. PMID: 12627942. 

Dulubova I, Yamaguchi T, Arac D, Li H, Huryeva I, Min SW, Rizo J, Sudhof TC. Convergence and divergence in the mechanism of SNARE binding by Sec1/Munc18-like proteins. Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):32-7. PMID: 12506202. 

Kulik V, Weyand M, Seidel R, Niks D, Arac D, Dunn MF, Schlichting I. On the role of alphaThr183 in the allosteric regulation and catalytic mechanism of tryptophan synthase. J Mol Biol. 2002 Dec 6; 324(4):677-90. PMID: 12460570. 

Antonin W, Dulubova I, Arac D, Pabst S, Plitzner J, Rizo J, Jahn R. The N-terminal domains of syntaxin 7 and vti1b form three-helix bundles that differ in their ability to regulate SNARE complex assembly. J Biol Chem. 2002 Sep 27; 277(39):36449-56. PMID: 12114520. 

Hafezparast M, Ball S, Nicholson SJ, Witherden A, Arac D, Broadway N, Saggerson D, Cooper E, Naase M, Gokhale S, Quant P, Lascelles C, Nickols C, Baker CS, Peters J, Martin JE, Fisher EM. A new mouse mutant, skijumper. Mamm Genome. 2002 Jul; 13(7):359-364. PMID: 12152619. 

Chen X, Tomchick DR, Kovrigin E, Araç D, Machius M, Südhof TC, Rizo J. Three-dimensional structure of the complexin/SNARE complex. Neuron. 2002 Jan 31; 33(3):397-409. PMID: 11832227. 

Witherden AS, Hafezparast M, Nicholson SJ, Ahmad-Annuar A, Bermingham N, Arac D, Rankin J, Iravani M, Ball S, Peters J, Martin JE, Huntley D, Hummerich H, Sergot M, Fisher EM. An integrated genetic, radiation hybrid, physical and transcription map of a region of distal mouse chromosome 12, including an imprinted locus and the ‘Legs at odd angles’ (Loa) mutation. Gene. 2002 Jan 23; 283(1-2):71-82. PMID: 11867214. 

Fernandez I, Araç D, Ubach J, Gerber SH, Shin O, Gao Y, Anderson RG, Südhof TC, Rizo J. Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine. Neuron. 2001 Dec 20; 32(6):1057-69. PMID: 11754837. 

Ubach J, Lao Y, Fernandez I, Arac D, Südhof TC, Rizo J. The C2B domain of synaptotagmin I is a Ca2+-binding module. Biochemistry. 2001 May 22; 40(20):5854-60. PMID: 11352720.